This web page was produced as an assignment for Genetics 564, an undergraduate course at UW-Madison .
Protein Interactions_______________________________________________________________________________
Proteins do not always function as a single unit: Many require interaction with other proteins in order to facilitate cellular and biological processes. Examples of such interactions include protein complex formation, and more transient interactions through binding that results in reversible changes like conformational changes and signal transduction. Protein interaction networks are investigated through through methods like yeast-two hybrid (Y2H) screening, affinity purification mass spectrometry, co-immunoprecipitation, and pull-down assays [1].
STRING is a database that provides data for known and predicted protein-protein interactions. The data are obtained through sources like experiments, other databases and text mining.
Proteins do not always function as a single unit: Many require interaction with other proteins in order to facilitate cellular and biological processes. Examples of such interactions include protein complex formation, and more transient interactions through binding that results in reversible changes like conformational changes and signal transduction. Protein interaction networks are investigated through through methods like yeast-two hybrid (Y2H) screening, affinity purification mass spectrometry, co-immunoprecipitation, and pull-down assays [1].
STRING is a database that provides data for known and predicted protein-protein interactions. The data are obtained through sources like experiments, other databases and text mining.
Proteins that Interact with DRD2_____________________________________________________________________________
According to STRING, examples of protein families that interact with DRD2 include the following:
Guanine nucleotide binding proteins (GNAI1, GNAI3, GNB1). Also known as G-proteins, they are involved in modulating or signal transduction in transmembrane systems. Melanocortin receptors (MC1R, MC4R, MC5R). Melanocortins are peptide hormones that activate melanocortin receptors. Like DRD2, these receptors are also members of the G-protein coupled receptor family. Somatostatin receptors (SSTR2, SSTR5). Somatostatins (SSTs) are a peptide hormones that inhibit release of certain hormones through association with their receptors. Somatostatin receptors are also members of the G-protein coupled receptor family. |
Regulators of G protein signalling (RGS10, RGS19).
These proteins inhibit signal transduction by inactivating G-proteins.
These proteins inhibit signal transduction by inactivating G-proteins.
Discussion______________________________________________________________________________________
The gene ontology terms obtained from STRING for the list of proteins that interact with DRD2 differ in different ways. The G-proteins, for example, are involved in intracellular signalling while PDYN, POMC, NPY, PENK and CRH are involved in extracellular signalling. However, all proteins in the list are involved in signal transduction.